ABSTRACT
Objective To establish an HPLC method for the determination of related substances of rabeprazole sodium.Methods The determination was performed on a Xtimate C18 column.The mobile phase consisted of 2 g/L ammonium acetate-acetonitrile (95:5)and 2 g/L ammonium-methanol(15:85), with linear gradient elution and the flow rate of 1.0 mL/min.Detection wavelength was 290 nm.Results Related substances were completely separated from the main constituent.The limit of detection of rabeprazole was 0.25 ng and the limit of quantification was 0.75 ng,which were 0.01% and 0.03% of test sample and met the detection.With the selected solvents, principal component could be extracted efficiently and good stability.The sample solution was not stable at room temperature.Conclusion The method is simple, rapid and accurate, and can be used to control the quality of rabeprazole sodium enteric-coated pellets.
ABSTRACT
Objective To screen the active component from Fructus Citri Sarcodactylis which have aortic vascular relaxant effect. Methods The active components from Fructus Citri Sarcodactylis were screened by combining vascular smooth muscle/cell membrane chromatography (VSM/CMC) and liquid chromatography/mass spectrometry (LC/MS) with pharmacological assay in vitro. Results Bergapten was the active component of Fructus Citri Sarcodactylis that functions in vascular. The pharmacological experiment showed that bergapten had vasodilating effect. The capacity factor of this compound on the VSM/CMC model was found to be significantly correlated with its pharmacological effect. Conclusion Association between compound and membrane protein (receptor) can be reflected by using the VSM/CMC model. VSM/CMC-offline-LC/MS technology can be used to quickly screening new active components from natural medicine.
ABSTRACT
A simple and reliable high performance liquid chromatography (HPLC) method has been developed for the simultaneous quantification of five major bioactive components in ‘Shu-Jin-Zhi-Tong' capsules (SJZTC), for the purposes of quality control of this commonly prescribed traditional Chinese medicine. Under the optimum conditions, excellent separation was achieved, and the assay was fully validated in terms of linearity, precision, repeatability, stability and accuracy. The validated method was applied successfully to the determination of the five compounds in SJZTC samples from different production batches. The HPLC method can be used as a valid analytical method to evaluate the intrinsic quality of SJZTC.